Zaleha Shafiei
Department of Craniofacial Diagnostics and Biosciences, Faculty of Dentistry, University Kebangsaan Malaysia, MalaysiaPublications
-
Research
Antibacterial and Antibiofilm Effects of Virgin Coconut and Virgin Olive Oils on Streptococcus sobrinus and Lactobacillus casei
Author(s): Nur Hafizah Hazmi, Zaleha Shafiei, Ahmad Shuhud Irfani Zakaria, S Nagarajan MP Sockalingam and Alida Mahyuddin*
Background: To determine the effect of virgin coconut and virgin olive oils on the growth of Streptococcus sobrinus and Lactobacillus casei using Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), to observe the effects of these oils on the morphology of Streptococcus sobrinus and Lactobacillus casei, to investigate the ant adherence and antibiofilm effects of these oils towards Streptococcus sobrinus and Lactobacillus casei in single- and dual-species biofilms. Materials and method: Broth microdilution technique was implemented to determine the MIC of the oils in a 96-wells microtiter plate, followed by MBC using the sub-cultured method. Chlorhexidine (0.12% CHX) and broth served as positive and negative controls respectively. All specimens that exhibited MIC were examined under a Transmission Electron Micr.. Read More»