Zahra Karami

Department of Biology, Shahid Bahonar University, Kerman, Iran

Publications

  • Commentary Article   
    Optimization of Fibrinolytic Enzyme from Bacillus KDm 99 Using Response Surface Method
    Author(s): Zahra Mirzaeia, Arastoo Badoei Dalfard* and Zahra Karami

    The purpose of this study is to employ the surface response approach to maximize the production of bacterial fibrinolytic enzyme. The Bacillus KDm99 bacterium stock was cultivated in nutrient broth, solid medium (nutrient agar-casein), and a special medium containing: peptone (0.5g), casein (0.1g), MgSO4 (0.02g), NaCl (0.02g), and KH2PO4 (0.05g). It was shown that the solid media had protease activity. The greatest protease activity was seen at a concentration of 50 microliters of enzyme with concentration (66.02 micro mole/min), 50 microliters of casein substrate with concentration (0.1%), 200 microliters of PBS-40 mm buffer with pH=8, and 300 microliters (Trichloroacetic acid)(5%) In order to optimize the bacterial growth environment for optimum enzyme synthesis, variables including supplies (carbon, nitrogen sources, metal ions, temperature, pH, agricultural residues, and animal fe.. Read More»

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