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The GC MS Study of Leaf Extract One Herbal Plant, Tarenna Asiatica (L)

Journal of Research in Medical and Dental Science
eISSN No. 2347-2367 pISSN No. 2347-2545

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Research Article - (2022) Volume 10, Issue 12

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The GC MS Study of Leaf Extract One Herbal Plant, Tarenna Asiatica (L)

Satheesh Kumar C1, Prabhu K2*, S Kalaivani3, A Franklin4, MRK Rao5, CS Janaki6 and Shruti Dinakaran6

*Correspondence: Dr. Prabhu K, Department of Anatomy, Sree Balaji Medical College and Hospital, Chennai, Tamil Nadu, India, Email:

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Abstract

To find the biomolecules present in the leaf aqueous extracts of one wild herbal plant, Tarenna asiatica (L.) by GC MS analysis. GC MS study of one leaf aqueous extracts of one wild herbal plant, Tarenna asiatica was subjected to GC MS analysis by standard procedures.

Results: It was observed that some important biomolecule present in the GC MS profile such as N-benzyl-2-phenethylamine, tridecanoic acid, methyl ester, pentanoic acid, phytol, 2-methylheptanoic acid, heptafluorobutyric acid, 2-naphthyl ester, 2(1H)-pyridinone, 1-[2-deoxy-3,5-bis-O-(4-methylbenzoyl)-beta-D-erythro-pentofuranosyl]- etc. had the medicinal roles which correspond well with medicinal roles of the plant as claimed ethno botanically and by scientific reports. The GC MS profile of T. asciatica clearly indicates the medicinal roles ascribed to it.

Keywords

Tarenna asiatica, GC MS, Aqueous, N-Benzyl-2-phenethylamine, Tridecanoic acid, Methyl ester, Pentanoic acid, Phytol, 2-Methylheptanoic acid, Heptafluorobutyric acid, 2-Naphthyl ester, 2(1H)-Pyridinone

Introduction

Tarenna asiatica (L) is a wild herb with many medicinal roles. Ethnobotanically is used for a treatment of a number of ailments such as wound healing, antidote, paralysis, and anti-inflammatory, for eye infection and to stop vomiting. Its antioxidant and antimicrobial activities have been reported by many authors Karthikkumaran, et al., Amutha, et al., Ramabharathi, et al. Anjanadevi, et al., Deborah, et al. have reported the anticancer activity of the fruit extract against human breast cancer. The present study is to know the biomolecules present in the leaf extract of this plant by GC MS analysis [1-5]. This knowledge could throw some light on the possible mechanisms for the medicinal roles of this plant. It is high time that herbal medicines should be thoroughly analysed in the light of modern medical parameters to establish their efficacy [6-24].

Materials and Methods

Tarenna asiatica plant was procured from the nearby hill at Chengalpattu and was identified by a qualified botanist from Madras University, Chennai. The leaves were collected and thoroughly washed and aqueous extracts was prepared. The extract was then dried and the powder obtained was subjected to GC MS analysis by standard procedure.

Instrument: Gas chromatography (Agilent: GC: (G3440A) 7890A. MS MS: 7000 triple Quad GCMS) was equipped with mass spectrometry detector.

Sample preparation: 100 micro lit samples dissolved in 1 ml of suitable solvents. The solution stirred vigorously using vortex stirrer for 10 seconds. The clear extract was determined using gas chromatography for analysis.

GC MS protocol: Column: DB5 MS (30 mm × 0.25 mm ID × 0.25 μm, composed of 5% phenyl 95% methyl poly siloxane), electron impact mode at 70 EV; helium (99.999%) was used as carrier gas at a constant flow of 1 ml/min injector temperature 280°C; auxiliary temperature: 290°C ion source temperature 280°C.

The oven temperature was programmed from 50°C (isothermal for 1.0 min), with an increase of 40°C/min, to 170°C C (isothermal for 4.0 min), then 10°C/min to 310°C (isothermal for 10 min) fragments from 45 to 450 Da. Total GC running time is 32.02 min. The compounds are identified by GC MS library (NIST and Wiley).

Results and Discussion

The results of GC MS study are shown in Table 1 and Figure 1.

Jrmds-graph

Figure 1: The GC MS graph of the leaf extract of Tarrena asiatica.

Reten. time Name of molecule Mol. formula Peak height Mol. mass Possible medicinal Roles
3.84 Hexane, 3,3-dimethyl- C8H18 7331565 114.1 Not Known
3.94 Bicyclo[3.2.0]hepta-2,6-diene C7H8 3860079 92.1 Not Known
4.45 N-benzyl-2-phenethylamine C15H17N 10042429 211.1 Anaphylactic, aryl amine-N-acetyl transferease Inhibitor, decrease nor epinephrine production, GABA-nergic, increase NK cell activity, Inhibit tumor necrosis factor, myo-neurostimulant, NADH oxidase inhibitor, CNS depressant
4.95 Acetoxy-3-methoxystyrene C11H12O3 4722140 192.1 Not known
5.83 Trimethylsilyloxycyclobutane C7H16OSi 3661847 144.1 Not known
6.18 tert-Butyldimethylsilyl acetate C8H18O2Si 3761014 174.1 Not known
6.26 Silane, [(1,1-dimethyl-2-propenyl)oxy]dimethyl- C7H16OSi 3257561 144.1 Oxytocic, oxytoxic
6.52 Silanol, trimethyl-, acetate C5H12O2Si 1109385 132.1 Not known
7.14 2-Cyclopentylethanol C7H14O 1645030 114.1 Not known
7.59 Tridecanoic acid, methyl ester C14H28O2 10830913 228.2 Catechol-o-methyl-transferase-inhibitor, catechol-o-methyltransferase-inhibitor, methyl donor, methyl guanidine inhibitor, acidifier, acidulant, arachidonic acid inhibitor, arachidonic acid inhibitor, increase aromatic amino acid decarboxylase activity, inhibit production of uric acid, urinary acidulant
7.76 Pentanoic acid C5H10O2 679733 102.1 Acidifer, arachidonic acid inhibitor, increase aromatic amino acid decarboxylase activity, increase uric acid production
8.74 1,3-Methanopentalene, octahydro- C9H14 1766693 122.1 Not known
8.82 Phytol C20H40O 12787752 296.3 Antimicrobial anti-inflammatory antioxidant diuretic
8.91 2-Methylheptanoic acid C8H16O2 419204 144.1 Acidifer, arachidonic acid inhibitor, increase aromatic amino acid decarboxylase activity, increase uric acid production
15.21 Trifluoroacetyl-lavandulol C12H17F3O2 8690639 250.1 Not Known
19.9 Phosphorus pentafluoride F5P 587768 126 Not known
19.98 1-Formyl-2,2,6-trimethyl-3-cis-(3-methylbut-2-enyl)-5-cyclohexene C15H24O 5380676 220.2 Not known
20.09 1,2-Benzenediol, O-(5-chlorovaleryl)-O-(2-methylbenzoyl)- C19H19ClO4 546676 346.1 Not known
20.21 Cyanogen bromide CBrN 802572 104.9 Cynogenic toxic
20.26 Heptafluorobutyric acid, 2-naphthyl ester C14H7F7O2 1142331 340 Acidifer, arachidonic acid inhibitor, increase aromatic amino acid decarboxylase activity, increase uric acid production
21.41 2(1H)-Pyridinone, 1-[2-deoxy-3,5-bis-O-(4-methylbenzoyl)-beta-D-erythro-pentofuranosyl]- C26H25NO6 689592 447.2 Acidifer, arachidonic acid inhibitor, increase aromatic amino acid decarboxylase activity, increase uric acid production
21.53 Carbamic acid, monoammonium salt CH6N2O2 1032531 78 Acidifer, arachidonic acid inhibitor, increase aromatic amino acid decarboxylase activity, increase uric acid production
21.65 10,10-Dimethyl-2,6-dimethylenebicyclo[7.2.0]undecan-5beta-ol C15H24O 7742701 220.2 Acidifer, arachidonic acid inhibitor, increase aromatic amino acid decarboxylase activity, increase uric acid production
21.72 Diazoprogesterone C21H30N4 6968207 338.2 Not known
21.92 6beta bicyclo[4.3.0]nonane, 5beta-iodomethyl-1beta-isopropenyl-4alpha, 5alpha-dimethyl-, C15H25I 7595792 332.1 Not known
22.02 Isophthalic acid, di(2-methylprop-2-en-1-yl) ester C16H18O4 1425098 274.1 Acidifer, arachidonic acid inhibitor, increase aromatic amino acid decarboxylase activity, increase uric acid production
22.3 Silane, diethylethoxy(2-methylpent-3-yloxy)- C12H28O2Si 5367612 232.2 Not known
22.38 1,3-Dioxolane-2-methanol C4H8O3 7084043 104 Not known
22.73 4-Methyl-2,4-bis(4-trimethylsilyloxyphenyl)pentene-1 C24H36O2Si2 9202952 412.2 Catachol-methyl transferase inhibitor, methyl donar, methyl guanidine inhibitor
22.74 1,3-Dimethyl-5-propyl-7-(propene-1-yl)adamantane C18H30 10822038 246.2 Not known
23.42 Tricyclo[3.3.1.1(3,7)]decanone, 4-iodo-, (1alpha, 3beta, 4alpha, 5alpha, 7beta) C10H13IO 3488103 276 Not known
23.75 1,3-Benzenediol, o-(2-methoxybenzoyl)-o'-ethoxycarbonyl- C17H16O6 1691039 316.1 Not known
24.55 1,2-Benzenediol, o-(4-methoxybezoyl)-o'-(5-chlorovaleryl)- C19H19ClO5 1718256 362.1 Not known

Table1: Indicating the molecules present in the GC MS analysis of Tarenna asiatica (L) with retention time, molecular formula, peak area, peak height and molecular mass and possible medicinal roles.

The medicinal roles of some of the molecules are mentioned as per Dr. Duke’s phytochemical data base. Most of the compounds such as N-benzyl-2- phenethylamine, tridecanoic acid, methyl ester, pentanoic acid, phytol, 2-methylheptanoic acid, heptafluorobutyric acid, 2-naphthyl ester, 2(1H)-pyridinone, 1-[2-deoxy-3,5- bis-O-(4-methylbenzoyl)-beta-D-erythro-pentofuranosyl]- etc. indicated similar medicinal roles such as catechol-omethyl- transferase-inhibitor,catechol-o-methyl transfera se inhibitor, methyl donor, methyl guanidine inhibitor, acidifier, acidulant, arachidonic acid inhibitor, arachidonic acid inhibitor, Increase aromatic amino acid decarboxylase activity, inhibit production of uric acid, urinary acidulate etc. It is interesting to note that the medicinal roles indicate mostly antioxidant, antibacterial antiinflammatory properties which auger well with the various reports on the ethno botanical medicinal roles of this plant.

The medicinal roles of some of the molecules such as hexane, 3, 3-dimethyl-, bicyclo[3.2.0]hepta-2,6-diene, 4- acetoxy-3-methoxystyrene, trimethylsilyloxycyclobutane, tert-butyldimethylsilyl acetate, silanol, trimethyl, acetate, 2-cyclopentylethanol, 1,3-methanopentalene, octahydro, trifluoroacetyl lavandulol, phosphorus pentafluoride, 1- formyl-2, 2,6-trimethyl-3-cis-(3-methylbut-2-enyl)-5- cyclohexene, 1,2-benzenediol, o-(5-chlorovaleryl)-O-(2-- (2-methylbenzoyl)-,Diazoprogesterone, 6.beta.Bicyclo [4.3.0]nonane, 5.beta.-iodomethyl-1.beta.-isopropenyl-4. alpha.,5.alpha.-dimethyl-,1, 3-Dioxolane-2-methanol, Tricyclo[3.3.1.1(3,7)]decanone, 4-iodo-,(1.alpha.3.beta., 4.alpha.,5.alpha.,7.beta.)-, 1,3-Benzenediol, o-(2- methoxybenzoyl)-o'-ethoxycarbonyl-, 1,2-Benzenediol, o- (4-methoxybezoyl)-o'-(5-chlorovaleryl)- are not reported yet which must be worked out.

Conclusion

From the above results and discussion it is clear that GC MS profile of aqueous extract of leaves of Tarenna asiatica (L) indicted the presence of some very important molecules having medicinal roles supporting the ethno botanical claims of this plant being an excellent medicinal plant.

Acknowledgements

The authors wish to acknowledge the help rendered by one and all in this work.

References

Author Info

Satheesh Kumar C1, Prabhu K2*, S Kalaivani3, A Franklin4, MRK Rao5, CS Janaki6 and Shruti Dinakaran6

1Department of Anatomy, Bharath Institute of Higher Education and Research, Melmaruvathur Adhiparasakthi Institute of Medical Sciences and Research, Melmaruvathur, Chennai, Tamil Nadu, India
2Department of Anatomy, Sree Balaji Medical College and Hospital, Chennai, Tamil Nadu, India
3Department of Anatomy, Vel’s Medical College and Hospital, Chennai, Tamil Nadu, India
4Department of Microbiology, CEO Anna Medical College, University of Technology, Mauritius, Port Louis, Mauritius
5Department of Anatomy, Amritha University, Old Mahabalipuram Road, Thiruporur, Tamil Nadu, India
6Department of Anatomy, Ayurvedic Medical Practioneer, Kottakkal Arya Vaidya Sala, Kerala, India
 

Citation: Satheesh Kumar C, Prabhu K, S Kalaivani, A Franklin, MRK Rao, CS Janaki, Shruti Dinakaran, The GC MS Study of Leaf Extract One Herbal Plant, Tarenna asiatica (L), J Res Med Dent Sci, 2022, 10 (12): 056-060

Received: 04-Oct-2022, Manuscript No. JRMDS-22-77105; , Pre QC No. JRMDS-22-77105(PQ); Editor assigned: 06-Oct-2022, Pre QC No. JRMDS-22-77105(PQ); Reviewed: 20-Oct-2022, QC No. JRMDS-22-77105; Revised: 21-Dec-2022, Manuscript No. JRMDS-22-77105(R); Published: 28-Dec-2022

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