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The Gas Chromatography Mass Spectroscopy Analysis of One Unani Drug, "Zimad Aouja"

Journal of Research in Medical and Dental Science
eISSN No. 2347-2367 pISSN No. 2347-2545

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Research Article - (2022) Volume 10, Issue 9

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The Gas Chromatography Mass Spectroscopy Analysis of One Unani Drug, "Zimad Aouja"

Hassan Mohammad M1, Janaki CS2, Rao MRK3*, Prabhu K4, Deepa K5, Franklin6 and Vijayalakshmi N7

*Correspondence: Rao MRK, Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India, Email:

Author info »

Abstract

The Unani drug, “Zimad Aouja”, is prescribed for joint sprains. The medicine was bought from Unani medicine supplier and was processed suitably for gas chromatography mass spectroscopic analysis. The profile showed metabolites, namely, 1-Hexadecyn-3-ol, 3,7,11,15-tetramethyl-, p-Menthane, 2,3-dibromo-8-phenyl-, Trichloroacetic acid, dodec-9-ynyl ester, Artumerone, Curlone, Benzenebutanal, gamma,4-dimethyl-, Methyl 4,7,10,13-hexadecatetraenoate, Tetradecanedioic acid, 6- Octadecenoic acid etc. which show promising medicinal roles. These medicinal roles could lead to the function of the medicine to cure the ailments for which this medicine is prescribed.

Keywords

GCMS, Zimad Aouja, Unani, Ar-tumerone, Curlone, Benzenebutanal, Tetradecanedioic acid, 6-Octadecenoic acid

Introduction

Zimad aouja is powder form of Unani medicine which contains mostly turmeric (Curcuma longa). When mixed with water it becomes and paste which is used to treat joint sprains. It can also be mixed with vinegar or hot water and spread on a cloth which is then applied to affected areas. It is imperative to establish the authenticity of alternative medicines such as Ayurveda, Sidhha and Unani systems as they are time tested and in use for centuries. The present workers have worked to scientifically evaluate the veracity of these medicine systems by latest techniques so that deeper knowledge of the mechanism of action of these medicines could be gained [1-19]. Not much work in this direction is reported as far as Zimad Aouja is concerned.

Materials and Methods

The drug, Zimad Aouja was bought from Unani medicine supplier and was suitably processed by standard procedures for the analysis.

Results

The Unani medicine Zimad Aouja gas chromatography mass spectroscopic profile and possible medicinal role of molecules is tabulated in Table 1.

Ret. Time Molecule Mol. Formula Mol. Mass % Peak Area Possible Medicinal Role
4.55 Cyclopentane, 1-pentyl-2-propyl- C13H26 182.2 1.46 Not known
4.61 Ribitol C5H12O5 152.1 1.04 Not known
4.82 Benzene, 1,3-bis(1,1-dimethylethyl)- C14H22 190.2 4.29 Not known
5.25 Dodecane, 1-fluoro- C12H25F 188.2 4.59 Not known
6.51 2-Methoxy-4-vinylphenol C9H10O2 150.1 1.15 Not known
7.53 Phenol, 2,4-bis(1,1-dimethylethyl)- C14H22O 206.2 1.56 Not known
7.99 1-Hexadecyn-3-ol, 3,7,11,15-tetramethyl- C20H38O 294.3 0.63 Oligosaccharide provider
8.26 p-Menthane, 2,3-dibromo-8-phenyl- C16H22Br2 372 0.9 Adrenalin pressor, Anti-cAMP-Phosphodiesterase, anticancer, antidote, antimitral valve prolapse
8.88 Trichloroacetic acid, dodec-9-ynyl ester C14H21C13O2 326.1 0.68 Arachidonic acid-inhibitor, increases aromatic amino Acid decarboxylase activity, inhibits production of uric Acid,
9.23 Ar-tumerone C15H20O 216.2 22.67 Arachidonic acid Inhibitor, arginine rich, aromatase inhibitor, Arterioconstrictor, aryl hydrocarbon hydroxylase inhibitor, arylamine-N-acetyltransferase inhibitor, increases aromatic amino acid decarboxylase activity, adrenaline pressor, alogenic, anti-cAMP phosphodiesterase, anticancer
9.35 Curlone C15H22O 218.2 5.2 Oligosaccharide provider
9.5 Benzenebutanal, gamma,4-dimethyl- C12H16O 176.1 0.59 PPAR-Gamma antagonistic
10.41 Methyl 4,7,10,13-hexadecatetraenoate C17H26O2 262.2 0.77 Catechol-o-methyl-transferase-inhibitor, methyl-donor, methyl-guanidine-inhibitor
11.3 3-Buten-2-one, 4-(4-hydroxy-3-methoxyphenyl)- C11H12O3 192.1 1.28 Not known
11.61 Methyl 16-hydroxy-hexadecanoate C17H34O3 286.3 0.97 17 beta hydroxysteroid dehydrogenase inhibitor, Aryl hydrocarbon hydroxylase inhibitor, testosterone hydroxylase inducer
13.78 Palmitoyl chloride C16H31ClO 274.2 0.95 Not known
14.3 Tetradecanedioic acid C14H26O4 258.2 3.87 Increases aromatic amino acid decarboxylase activity, inhibit production of uric acid,
14.9 Decanamide, N-(2-hydroxyethyl)- C12H25NO2 215.2 0.9 Not known
16.22 6-Octadecenoic acid C18H34O2 282.3 5.47 Increases aromatic amino acid decarboxylase activity, inhibit production of uric acid
16.82 9-Octadecenoic acid, (E)- C18H34O2 282.3 16.84 Not known
17.07 Butyl 9,12,15-octadecatrienoate C22H38O2 334.3 15.63 Not Known

Table 1: Indicates the retentions values, types of possible compound, their molecular formulae, molecular mass, peak area and their medicinal roles of each compound as shown in the GC MS profile of Zimad Aouja.

Figure 1 shows the gas chromatography mass spectroscopic of the Unani medicine Zimad Aouja. The identification of metabolites was accomplished by comparison of retention time and fragmentation pattern with mass spectra in the NIST spectral library stored in the computer software (version 1.10 beta, Shimadzu) of the GC-MS along with the possible pharmaceutical roles of each bio molecule as per Dr. Duke’s phytochemical and ethnobotanical data base (National Agriculture Library, USA) and others as shown in Table 1 [20].

medical-dental-science

Figure 1: Indicates the Gas chromatography mass spectroscopic profile of Zimad Aouja.

Discussion

The gas chromatography mass spectroscopic profile of Zimad Aouja showed compounds, namely, 1-Hexadecyn-3-ol, 3,7,11,15-tetramethyl-, p-Menthane, 2,3-dibromo-8-phenyl-, Trichloroacetic acid, dodec-9-ynyl ester, Ar-tumerone, Curlone, Benzenebutanal, gamma,4-dimethyl-, Methyl 4,7,10,13-hexadecatetraenoate, Tetradecanedioic acid, 6-Octadecenoic acid etc. which have important medicinal roles as shown in Table 1. These medicinal roles must be attributed to the medicinal role of Zimad aouja which is prescribed pain relief due to sprains etc.

Conclusion

It could be summarized from the results and discussion that Zimad Aouja does contain important biomolecules which provides a clue to its prescription for the ailments it is given. Some of the molecules shown in the GCMS profile for which the medicinal roles are not known could be further probed.

Acknowledgement

The authors thankfully acknowledge the support of all the people and organizations.

References

Author Info

Hassan Mohammad M1, Janaki CS2, Rao MRK3*, Prabhu K4, Deepa K5, Franklin6 and Vijayalakshmi N7

1Department of Anatomy, Northern Borders University, Arar, Saudi Arabia
2Department of Anatomy, Bhaarath Medical College, Chennai, Tamilnadu, India
3Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India
4Department of Anatomy, Sree Balaji Medical College and Hospital, Chennai, Tamil Nadu, India
5Department of Anatomy, Quest International University, IPOH Perak, Malaysia
6Dept of Microbiology, CEO Anna Medical College, Mauritius, Montagne Blanche, Island, Mauritius
7Department of Chemical and Biotechnology, SASTRA (Deemed to be University), Thanjavur, Tamil Nadu, India
 

Received: 04-Jul-2022, Manuscript No. JRMDS-22-57371; , Pre QC No. JRMDS-22-57371; Editor assigned: 07-Jul-2022, Pre QC No. JRMDS-22-57371; Reviewed: 21-Jul-2022, QC No. JRMDS-22-57371; Revised: 05-Sep-2022, Manuscript No. JRMDS-22-57371; Published: 12-Sep-2022

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