Research Article - (2022) Volume 10, Issue 9
The Gas Chromatography Mass Spectroscopy Analysis of One Unani Drug, "Sherbath E-Bailphal"
Hassan mohammad M1, Janaki CS2, Rao MRK3*, Prabhu K4, Deepa K5, Franklin6 and Vijayalakshmi N7
*Correspondence: Rao MRK, Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India, Email:
Abstract
The Unani medicine, Sherbath e-bailphal a health drink made of the pulp of Bail phal, or Bilva (Aegle marmelos L). This fruit has many medicinal values and can be taken as a health drink. The medicine was bought from a Unani medicine supplier and was processed suitably to be analysed by GC MS process. The pofile showed many molecules, namely, 8-Hydroxy-2, 2, 8-trimethyldeca-5,9-dien-3-one, 5-Hydroxymethylfurfural, 1,15-Pentadecanedioic acid, Methyl 2-hydroxyoctadeca-9,12,15-trienoate, Methyl 3-cis,9-cis,12-cis-octadecatrienoate, (E,Z,Z)-2,4,7-Tridecatrienal, Hexadecanoic acid, cyclohexyl ester etc. which could play the medicinal roles of Sherbath Ibilphal.
Keywords
GC MS, Unani, Sherbath e-bailphal, 8-Hydroxy-2, 2, 8-trimethyldeca-5,9-dien-3-one, 5-Hydroxymethylfurfural, 1,15-Pentadecanedioic acidIntroduction
Sherbath e bailphal is the health drink made of the pulp of Bail phal, or Bilva (Aegle marmelosL).Bilva has many medicinal roles [1]. Sherbath e-bailphal is taken as health drink particularly during summer as a coolant. The present workers have worked to scientifically evaluate the veracity of these medicine systems by latest techniques so that deeper knowledge of the mechanism of action of these medicines could be gained [2-20]. The present study in one step further in this endeavour. Not much work in this direction is reported as far as “Sherbath e-bailphal” is concerned.
Materials and Methods
The medicine Sherbath e-bailphal was bought from Unani medicine supplier and was suitably processed by standard procedures and the GC-MS analysis was performed.
Results
The gas chromatography mass spectroscopic profile of Sherbath e-bailphal and possible medicinal role of each molecule is tabulated in Table 1.
Ret. Time | Molecule | Mol. Formula | Mol. Mass | % peak Area | Possible Medicinal Role |
---|---|---|---|---|---|
3.75 | 8-Hydroxy-2,2,8-trimethyldeca-5,9-dien-3-one | C13H22O2 | 210.2 | 0.23 | 17 beta hydroxysteroid dehydrogenase inhibitor |
4.33 | 10-Chlorodecyl isobutyl carbonate | C15H29ClO3 | 292.2 | 0.24 | Not known |
4.81 | Benzene, 1,3-bis(1,1-dimethylethyl)- | C14H22 | 190.2 | 1.76 | Not known |
6.31 | 5-Hydroxymethylfurfural | C6H6O3 | 126 | 4.08 | It is reported to stop neuron apoptosis |
8.03 | Methylparaben | C8H8O3 | 152 | 19.3 | Not known |
10.79 | 13-Tetradecenal | C14H26O | 210.2 | 0.24 | Not known |
13.78 | Palmitoyl chloride | C16H31ClO | 274.2 | 0.82 | Not known |
14.29 | 1,15-Pentadecanedioic acid | C15H28O4 | 272.2 | 2.61 | Arachidonic acid-Inhibitor, Increase Aromatic Amino Acid Decarboxylase Activity, Inhibit Production of Uric Acid, |
16.82 | 9-Octadecenoic acid, (E)- | C18H34O2 | 282.3 | 12.37 | Not Known |
17.06 | Methyl 2-hydroxy-octadeca-9,12,15-trienoate | C19H32O3 | 308.2 | 12.8 | 17 beta hydroxysteroid dehydrogenase inhibitor, aryl hydrocarbon hydroxylase inhibitor, testosterone hydroxylase inducer, Catechol-O-Methyl-Transferase-Inhibitor |
17.5 | Methyl 3-cis,9-cis,12-cis-octadecatrienoate | C19H32O2 | 292.2 | 0.21 | Catechol-O-Methyl-Transferase-Inhibitor, Methyl-Donor, Methyl-Guanidine-Inhibitor |
17.75 | (E,Z,Z)-2,4,7-Tridecatrienal | C13H20O | 192.2 | 0.52 | Provides zinc, anticancer, antidote, Cancer-Preventive, Cytochrome-P450-2E1-Inhibitor, Decreases C telopeptide excretion, decrease deoxypyridinoline excreation, decreses endothelial leukocyte adhesion, decrease endothelial platelet adhesion, decrease epinephrine production, decreases oxalate excretion. |
29.39 | Hexadecanoic acid, cyclohexyl ester | C22H42O2 | 338.3 | 37.16 | Increase Aromatic Amino Acid Decarboxylase Activity, Inhibit Production of Uric Acid |
Table 1: Indicates the retentions values, types of possible compound, their molecular formulae, molecular mass, peak area and their medicinal roles of each compound as shown in the GC MS profile of Sherbath e bailphal.
Figure 1 depicts gas chromatography mass spectroscopic profile of Sherbath e-bailphal. The metabolites were identified by comparison with NIST spectral library and the possible pharmaceutical roles of each bio molecule as per National Agriculture Library, USA and others as shown in Table 1 [21].
Figure 1: Shows the gas chromatography mass spectroscopic profile of Sherbath e bailphal.
Discussion
The gas chromatography mass spectroscopic profile of Sherbath e-bailphal shows compounds, namely, 8-Hydroxy-2, 2, 8-trimethyldeca-5,9-dien-3-one, 5-Hydroxymethylfurfural, 1,15-Pentadecanedioic acid, Methyl 2-hydroxy-octadeca-9,12,15-trienoate, Methyl 3-cis,9-cis,12-cis-octadecatrienoate, (E,Z,Z)-2,4,7-Tridecatrienal, Hexadecanoic acid, cyclohexyl ester etc. which have medicinal roles, as shown in Table 1. These molecules could be contributing to the cure of diseases for which Sherbath e bailphal is prescribed.
Conclusion
It could be summarized from the results and discussion that Sherbath e-Bailphal does contain important biomolecules which provides a clue to its prescription for the ailments it is given. Some of the molecules shown in the GC MS profile for which the medicinal roles are not known could be further probed.
Acknowledgements
The authors thankfully acknowledge the support of all the people and organizations.
References
- Rahman S, Pravin R. Therapeutic potential of Aegle marmelos). An overview. Asian Pac J of Trop Dis 2014; 4:71-77.
- Rao MRK, Philip S, Kumar MH, et al. GC-MS analysis, antimicrobial, antioxidant activity of an Ayurvedic medicine, Salmali Niryasa. J Chem Pharm Res 2015; 7:131-139.
- Sivakumaran G, Prabhu K, Rao MRK, et al. Gas chromatographyâ??mass spectrometry analysis of one ayurvedic oil, Anu thailam. DIT 2019; 11:2675-2678.
- Sivakumaran G, Prabhu K, Rao MRK, et al. Gas chromatographyâ??mass spectrometry analysis of one ayurvedic oil, Ksheerabala Thailam. DIT 2019; 11: 2661-2665.
- Sivakumaran G, Prabhu K, Rao MRK, et al. Gas chromatographyâ??mass spectrometry analysis of one Ayurvedic oil, Triphaladi Thailam. DIT 2019; 11:2679-2683.
- Narayanan G, Prabhu K, Rao MRK, et al. Gas chromatographyâ??mass spectrometry analysis of one Ayurvedic medicine, Drakshadi Kashayam. DIT 2019; 11: 2652-2656.
- Narayanan G, Prabhu K, Rao MRK, et al. Gas chromatographyâ??mass spectrometry analysis of one ayurvedic medicine, Kutajarishtam. DIT 2019; 11:2666-2669.
- Narayanan G, KPrabhu K, Rao MRK, et al. Gas chromatographyâ??mass spectrometry analysis of one Ayurvedic antiobesity medicine, Lohasava. DIT 2019; 11:2670-2674.
- Kumar MH, Prabhu K, Rao MRK, et al. Gas chromatography/mass spectrometry analysis of one Ayurvedic skin oil, Eladi Kera Thailam. DIT 2019; 11:2657-2660.
- Mohammad H, Prabhu K, Rao MRK, et al. The GC MS study of one Ayurvedic Pain relieving OIL "Mahamasha thailam". Drug Discov Today 2019; 12:1524-1527.
- Mohammad H, Prabhu K, Rao MRK, et al. The GC MS study of one Ayurvedic Pain relieving oil "Karpooradi thailam", Drug Invention Today, 2019; 12:1542-1546.
- Prabhu J, Prabhu K, Chaudhury A, et al. Neuro protective role of Saraswatharishtam on Scopolamine induced memory impairment in animal model. Pharmacogn J 2020; 12:465-472.
- Prabhu K, Rao MRK, AK Bharath, et al. The GC MS study of one Ayurvedic Rasayana formulation Narasimha Rasayanam. DIT 2020; 13:658-662.
- Prabhu K, Rao MRK, Vishal SK, et al. GC MS study of one Ayurvedic Rasayana drug, Dhanwantari Rasayanam. DIT 2020; 14:783-786.
- Sharmila D, A Poovarasan, Pradeep E, et al. GC MS analysis of one Ayurvedic formulation, Sitopaladi. RJPT 2021; 14:911-915.
- Narayanan G, Prabhu K, Chaudhuri A, et al. Cardio protective role of Partharishtam on isoproterenol induced myocardial infarction in animal model. Pharmacogn J 2021; 13:591-595.
- Kalivannan J, Janaki CS, Rao MRK, et al. The GC MS astudy of one ayurvedic formulation, Chandanasavam. Ind J of Nat Sci 2021; 12:33671-33676.
- Akshaya SR, Kalaivani S, Prabhu K, et al. The GC MS study of one Ayurvedic churnam, Avalgujabijadi churnam. Ind J of Nat Sci 2021; 12:34395-34402.
- Subbiah AJ, Kavimani M, Rao MRK, et al. The GC MS study of one Ayurvedic. Formulation, Pushyanuga churnam. Ind J Nat Sci 2021; 12:35757-357-366.
- Yuvaraj R, Vijayakumar S, Rao MRK, et al. The GC MS study of one Ayurvedic medicine Pippalyasavamâ??. Ind J of Nat Sci 2021; 12:35612-35618.
- Duke, James A. Dukeâ??s Phytochemcial and Ehnobotanical Databases. U.S. Department of Agriculture, Agricultural Research Service. Ag Data Commons, U.S, 2021.
Author Info
Hassan mohammad M1, Janaki CS2, Rao MRK3*, Prabhu K4, Deepa K5, Franklin6 and Vijayalakshmi N7
1Department of Anatomy, Northern Borders University, Arar, Saudi Arabia2Department of Anatomy, Bhaarath Medical College, Chennai, Tamilnadu, India
3Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India
4Department of Anatomy, Sree Balaji Medical College and Hospital, Chennai, Tamil Nadu, India
5Department of Anatomy, Quest International University, IPOH Perak, Malaysia
6Dept of Microbiology, CEO Anna Medical College, Mauritius, Montagne Blanche, Island, Mauritius
7Department of Chemical and Biotechnology, SASTRA (Deemed to be University), Thanjavur, Tamil Nadu, India
Received: 02-Jul-2022, Manuscript No. JRMDS-22-57369; , Pre QC No. JRMDS-22-57369; Editor assigned: 05-Jul-2022, Pre QC No. JRMDS-22-57369; Reviewed: 19-Jul-2022, QC No. JRMDS-22-57369; Revised: 02-Sep-2022, Manuscript No. JRMDS-22-57369; Published: 08-Sep-2022