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The Gas Chromatography Mass Spectroscopy Analysis of One Unani Drug, "Muffarah Ahmedi"

Journal of Research in Medical and Dental Science
eISSN No. 2347-2367 pISSN No. 2347-2545

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Research Article - (2022) Volume 10, Issue 9

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The Gas Chromatography Mass Spectroscopy Analysis of One Unani Drug, "Muffarah Ahmedi"

Hassan Mohammad M1, Janaki CS2, Rao MRK3*, Prabhu K4, Deepa K5, Franklin6 and Vijayalakshmi N7

*Correspondence: Rao MRK, Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India, Email:

Author info »

Abstract

The Unani medicine, Muffarah Ahmedi, which is prescribed for duodenal and peptic ulcers, was subjected to gas chromatography mass spectroscopic analysis. The medicine was bought procured from Unani medicine supplier and was processed suitably for analysis. The profile results indicated the availability of many compounds, namely, trans-2-methyl-4-n-pentylthiane, S,Sdioxide,PhenylethylAlcohol,trans-3-Methyl-2-n-propylthiophane,5-Hydroxymethylfurfural, Tetradecanedioic acid, 6-Octadecenoic acid, trans-13-Octadecenoic acid, Methyl 2-hydroxy-octadeca-9,12,15-trienoate, which correspond well with the medicinal role of this medicine.

Keywords

GCMS, Unani, Muffarah Ahmedi, Phenylethyl alcohol, Trans-3-Methyl-2-n-propylthiophane, 5-Hydroxymethylfurfural

Introduction

Muffarah Ahmedi is a Unani medicine used to treat duodenal and gastric ulcers and supposed to work as coolant. Its ingredients constitute of Thabasheer (Bamboo extracts), Sandal-e-sufeed (White sandal wood), Amila (Phyllanthus embelica), Gul e-surkh (Rose flower), Kishneez (Coriandrum sativum L.) and Khande sufeed (White sugar). It is imperative to establish the authenticity of alternative medicines such as Ayurveda, Sidhha and Unani systems as they are time tested and in use for centuries. The present workers have worked to scientifically evaluate the veracity of these medicine systems by latest techniques so that deeper knowledge of the mechanism of action of these medicines could be gained [1-19].The present study in one step further in this endeavour. Not much work in this direction is reported on the medicinal role of this medicine.

Materials and Methods

Muffarah Ahmedi was bought from a Unani medicine vendor at chennai. The medicine was suitably processed by standard procedures and the GC-MS analysis was performed.

Results

Muffarah Ahmedi gas chromatography mass spectroscopy profile and possible medicinal role of each molecule indicated in the GC MS profile is tabulated in Table 1.

Ret. Time Molecule Mol. Formula Mol. Mass % peak Area Possible Medicinal Roles
4.41 trans-2-methyl-4-n-pentylthiane, S,S-dioxide C11H22O2S 218.1 1.8 Nitric oxide synthase inhibitor, Glutathione-S-Transferase-Inhibitor, Myo-neuro stimulant, Nitric Oxide scavenger, stimulates norepinephrine production, Stimulates Sympathetic nervous system, Catechol-O- Methyl transferase inhibitor, known as smart drug, Adrenocortical stimulant, Decreases Glutamate Oxaloacetate Transaminase, Decreases Glutamate Pyruvate Transaminase, Glucosyl-Transferase-Inhibitor, Glutathione-S-Transferase-Inhibitor, Increases Glyoxalate Transamination, Reverse-Transcriptase-Inhibitor, Transdermal
4.64 Phenylethyl Alcohol C8H10O 122.1 6.17 Alcohol dehydrogenase inhibitor, Alcohol detoxicant
4.94 trans-3-Methyl-2-n-propylthiophane C8H16S 144.1 0.95 Catechol-O-Methyl-Transferase-Inhibitor, increases Glutathione-S-Transferase (GST) activity, decreases glutamate oxaloacetate transaminase, decreases glutamate pyruvate transaminase, glucosyl-transferase-inhibitor, glutathione-S-transferase-inhibitor, increases glyoxalate transamination, reverse-transcriptase-Inhibitor, GABAergic, increased NK cell activity, inhibits production of tumour necrosis factor, Myo-Neuro-stimulator
5.26 Dodecane, 1-fluoro- C12H25F 188.2 5.48 Not known
6.31 5-Hydroxymethylfurfural C6H6O3 126 12.72 It is reported to stop neuron apoptosis
6.39 Triallylmethylsilane C10H18Si 166.1 0.76 Not known
8.03 Methylparaben C8H8O3 152 20.26 Not known
9.28 Isobutyl 4-hydroxybenzoate C11H14O3 194.1 11.6 Not known
13.78 Palmitoyl chloride C16H31ClO 274.2 0.68 Not known
14.3 Tetradecanedioic acid C14H26O4 258.2 1.77 acidifier, acidulant, arachidonic acid-inhibitor, increases aromatic amino acid decarboxylase activity, inhibits Production of Uric Acid
16.22 6-Octadecenoic acid C18H34O2 282.3 5.26 acidifier, acidulant, arachidonic acid-inhibitor, increases aromatic amino acid decarboxylase activity, inhibits production of uric Acid
16.82 trans-13-Octadecenoic acid C18H34O2 282.3 10.52 Catechol-O-methyl-transferase-Inhibitor, increases Glutathione-S-Transferase (GST) activity, decreases glutamate oxaloacetate transaminase, decreases glutamate pyruvate transaminase, tlucosyl-transferase-Inhibitor, glutathione-S-transferase-Inhibitor, increases glyoxalate transamination, Reverse-transcriptase-inhibitor, transdermal, acidifier, arachidonic acid Inhibitor, increases aromatic amino acid decarboxylase activity, inhibits production of uric acid, urine acidifier
17.07 Methyl 2-hydroxy-octadeca-9,12,15-trienoate C19H32O3 308.2 10.63 17 beta hydroxyl steroid dehydrogenase inhibitor, aryl hydrocarbon hydroxylase inhibitor, testosterone hydroxylase inducer, Catechol-O-methyl-transferase-inhibitor, methyl donar, methyl guanidine inhibitor

Table 1: Indicates the retentions values, types of possible compound, their molecular formulae, molecular mass, peak area and their medicinal roles of each compound as shown in the GC MS profile of Muffarah Ahmedi.

Figure 1 shows gas chromatography mass spectroscopy profile of Muffarah Ahmedi. The identification of metabolites was compared with NIST spectral library and the possible pharmaceutical roles of each bio molecule was referred with National Agriculture Library, USA and others as shown in Table 1 [20].

medical-dental-science

Figure 1: Indicates the gas chromatography mass spectroscopic profile of Muffarah Ahmedi.

Discussion

Muffarah Ahmedi contained some compounds, namely, trans-2-methyl-4-n-pentylthiane, S,S-dioxide, phenylethyl alcohol, trans-3-Methyl-2-n-propylthiophane, 5-hydroxymethylfurfural, tetradecanedioic acid, 6-octadecenoic acid, trans-13-octadecenoic acid, methyl 2-hydroxy-octadeca-9,12,15-trienoate etc. which were reported to have far reaching medicinal roles that could support the role of Muffarah Ahmedi as a good medicine for duodenal and peptic ulcers.

Conclusion

It could be summarized from the results and discussion that Muffarah Ahmedi does contain important biomolecules which provides a clue to its prescription for the ailments it is given. It will be of interest to probe into the medicinal roles of many compound present in Muffarah Ahmedi for which reports are not available.

Acknowledgements

The authors thankfully acknowledge the support of all the people and organizations.

References

Author Info

Hassan Mohammad M1, Janaki CS2, Rao MRK3*, Prabhu K4, Deepa K5, Franklin6 and Vijayalakshmi N7

1Department of Anatomy, Northern Borders University, Arar, Saudi Arabia
2Department of Anatomy, Bhaarath Medical College, Chennai, Tamilnadu, India
3Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India
4Department of Anatomy, Sree Balaji Medical College and Hospital, Chennai, Tamil Nadu, India
5Department of Anatomy, Quest International University, IPOH Perak, Malaysia
6Deparmentt of Microbiology, CEO Anna Medical College, Mauritius, Montagne Blanche, Island, Mauritius
7Department of Chemical and Biotechnology, SASTRA (Deemed to be University), Thanjavur, Tamil Nadu, India
 

Citation: Hassan mohammad M, Janaki CS, Rao MRK, Prabhu K, Deepa K, Franklin, Vijayalakshmi N,The Gas Chromatography Mass Spectroscopy Analysis of One Unani Drug, “Muffarah Ahmedi”, J Res Med Dent Sci, 2022, 10 (9): 000-000.

Received: 01-Jul-2022, Manuscript No. JRMDS-22-57365; , Pre QC No. JRMDS-22-57365; Editor assigned: 04-Jul-2022, Pre QC No. JRMDS-22-57365; Reviewed: 18-Jul-2022, QC No. JRMDS-22-57365; Revised: 01-Sep-2022, Manuscript No. JRMDS-22-57365; Published: 07-Sep-2022

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