GET THE APP
The Gas Chromatography Mass Spectroscopic Study of One Unani Drug, "Apk Abraisham"

Journal of Research in Medical and Dental Science
eISSN No. 2347-2367 pISSN No. 2347-2545

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - (2022) Volume 10, Issue 8

View PDF Download PDF

The Gas Chromatography Mass Spectroscopic Study of One Unani Drug, "Apk Abraisham"

Hassan Mohammad M1, Janaki CS2, Rao MRK3*, Prabhu K4, Deepa k5, Franklin6 and Vijayalakshmi N7

*Correspondence: Rao MRK, Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India, Email:

Author info »

Abstract

This work deals with the gas chromatography mass spectroscopic study of one Unani medicine, Apk Abraisham, which is prescribed for Ano-rectal disorders as well as nerve related problems. The medicine was procured from a standard Unani medicine vendor at Chennai and was processed suitably to be analysed by GC MS process. The results indicated the presence of many molecules such as trans-2,4-Dimethylthiane, S,S-dioxide, trans-2-methyl-4-n-pentylthiane, S,S-dioxide, Cyclopentane, 1-pentyl-2-propyl-, Terpinen-4-ol, alpha-Terpineol, Pivalic acid, 2-tetrahydrofurylmethyl ester, Propanoic acid, 2,2-dimethyl-2-ethylhexyl ester, trans-2,4-Dimethylthiane, S,S-dioxide, 3-Cyclohexene-1-ethanol, beta, 4-dimethyl-, Trichloroacetic acid, undecyl ester, Carbonochloridic acid, decyl ester, Sulfurous acid, butyl undecyl ester, Phthalic acid, octyl tridec-2-yn-1-yl ester etc. which show promising medicinal roles. These medicinal roles could lead to the cure of the ailments for which this medicine is prescribed.

Keywords

Apk Abraisham, Unani, GC MS, Sulfurous acid, Butyl undecyl ester, Trans-2,4-Dimethylthiane, S,S-dioxide

Introduction

Apk Abraisham is a Unani medicine prescribed for a number of ailments like haemorrhoids, piles, fistula, painful defecation, rectal bleeding, fissures and joint and muscular disorders. It also claimed to improve concentration, correct hyperactive disorder, helps in Parkinson’s diseases, Alzheimer disease, dementia and psychological issues. It is imperative to establish the authenticity of alternative medicines such as Ayurveda, Sidhha and Unani systems as they are time tested and in use for centuries. The present workers have worked to scientifically evaluate the veracity of these medicine systems by latest techniques so that deeper knowledge of the mechanism of action of these medicines could be gained [1-19]. The present study in one step further in this endeavour. Not much work in this direction is reported as far as Apk Abraisham is concerned.

Materials and Methods

Apk Abraisham was procured from Unani medicine vendor at Chennai. The medicine was suitably processed by standard procedures and the GC-MS analysis was performed.

Results

The possible medicinal role of each molecule indicated in the gas chromatography mass spectroscopy profile is tabulated in Table 1.

Ret. Time Molecule Mol. Formula Mol. Mass % Peak Value Possible medicinal role
3.96 trans-2,4-Dimethylthiane, S,S-dioxide C7H14O2S 162.1 0.86 Glutathione-S-Tansferase inhibitor, increases glutathinone-S-transferase (GST) activity, decreases oxaloacetate transaminase activity, Catechol-O-Methyl-Transferase-Inhibitor, Decreases Glutamate Oxaloacetate Transaminase, Decreases Glutamate PuruvateTransaminase, Glucosyl-Transferase inhibitor, increases glyoxalate transamination, reverse transcriptase inhibitor, transdermal, smart drug, adrenocortical stimulant
4.16 Tremorine C12H20N2 192.2 0.92 Not known
4.41 trans-2-methyl-4-n-pentylthiane, S,S-dioxide C11H22O2S 218.1 6.08 Glutathione-S-Transferase-Inhibitor, Catechol-O-Methyl-Transfearse inhibitor, Myo-neuro-stimulator, Nitric Oxide Synthatase inhibitor, NO scavenger, Stimulates Morepinephrine production, Stimulates Sympathetic nervous system, decrease glutamate oxaloacetate transaminase, decrease glutamine pyruvate transaminase, Glycosyl transferase inhibitor, increases glyoxalate transamination, reverse transcriptase inhibitor, smart drug, adrenal supporter
4.48 Cyclopentane, 1-pentyl-2-propyl- C13H26 182.2 9.67 Catechol-O-Methyl-Transfearse inhibitor
4.55 Terpinen-4-ol C10H18O 154.1 13.09 Oligosaccharide provider
4.75 .alpha.-Terpineol C10H18O 154.1 3.35 HIF 1 alpha inhibitor, Ikappa B alpha phosphorylation inhibitor, increases alpha mannosidae activity, Interleukine 1 alpha inhibitor, TNF alpha inhibitor
4.81 Benzene, 1,3-bis(1,1-dimethylethyl)- C14H22 190.2 23.09 Not known
5.25 Dodecane, 1-fluoro- C12H25F 188.2 20.24 Not known
5.42 1-Cyclohexylethanol, trifluoroacetate C10H15F3O2 224.1 0.94 Not known
5.47 Pivalic acid, 2-tetrahydrofurylmethyl ester C10H18O3 186.1 0.93 Arachidonic acid-Inhibitor, Increases Aromatic Amino Acid Decarboxylase Activity
5.69 Propanoic acid, 2,2-dimethyl-, 2-ethylhexyl ester C13H26O2 214.2 1.38 Arachidonic acid-Inhibitor, Increase Aromatic Amino Acid Decarboxylase Activity
5.86 trans-2,4-Dimethylthiane, S,S-dioxide C7H14O2S 162.1 0.85 Glutathione-S-Tansferase inhibitor, increases glutathinone-S-transferase (GST) activity, decreases oxaloacetate transaminase activity, Catechol-O-Methyl-Transferase-Inhibitor, Decrease Glutamate Oxaloacetate Transaminase, Decrease Glutamate PuruvateTransaminase, Glucosyl-Transferase inhibitor, increases glyoxalate transamination, reverse transcriptase inhibitor, transdermal, smart drug, adrenocortical stimulant
6.05 3-Cyclohexene-1-ethanol, beta.,4-dimethyl- C10H18O 154.1 0.74 Ethanol absorption inhibitor, ethanolytic, 17 beta hydroxysteroid dehydrogenase inhibitor, anti- amyloid beta, anti-amyloid beta, anti TGF beta, beta receptor agonist, bête andrenergic receptor blocker, beta blocker, beta galactosidase inhibitor, beta glucuronidase inhibitor, ER beta binder
6.18 Trichloroacetic acid, undecyl ester C13H23Cl3O2 316.1 1.08 Arachidonic acid-Inhibitor, Increase Aromatic Amino Acid Decarboxylase Activity,
6.39 Carbonochloridic acid, decyl ester C11H21ClO2 220.1 1.63 Arachidonic acid-Inhibitor, Increase Aromatic Amino Acid Decarboxylase Activity
7.53 Phenol, 2,4-bis(1,1-dimethylethyl)- C14H22O 206.2 4.48 Not known
7.66 5-Isopropenyl-2-methylcyclopent-1-enecarboxaldehyde C10H14O 150.1 1.17 Not known
8.42 Sulfurous acid, butyl undecyl ester C15H32O3S 292.2 3.74 Arachidonic acid-Inhibitor, Increase Aromatic Amino Acid Decarboxylase Activity,
9.1 Diethyl Phthalate C12H14O4 222.1 1.3 Not known
18.21 Phthalic acid, octyl tridec-2-yn-1-yl ester C29H44O4 456.3 1.08 Arachidonic acid-Inhibitor, Increase Aromatic Amino Acid Decarboxylase Activity,

Table 1: Indicates the retentions values, types of possible compound, their molecular formulae, molecular mass, peak area and their medicinal roles of each compound as shown in the GC MS profile of Apk Abraisham.

Figure 1 represents the GC-MS profile of the Unani medicine Apk Abraisham. The molecules were identified by using NIST spectral Library and the possible pharmaceutical roles of each bio molecule was as per (National Agriculture Library, USA) and others as shown in Table 1 [20].

medical-denta-science

Figure 1: Indicates the GC MS profile of Apk Abraiisham.

Discussion

Apk Abraisham profile showed some important compounds such as, trans-2,4-Dimethylthiane, S,S-dioxide, trans-2-methyl-4-n-pentylthiane, S,S-dioxide, Cyclopentane, 1-pentyl-2-propyl-, Terpinen-4-ol, alpha-Terpineol, Pivalic acid, 2-tetrahydrofurylmethyl ester, Propanoic acid, 2,2-dimethyl-, 2-ethylhexyl ester, trans-2,4-Dimethylthiane, S,S-dioxide, 3-Cyclohexene-1-ethanol, beta, 4-dimethyl-, Trichloroacetic acid, undecyl ester, Carbonochloridic acid, decyl ester, Sulfurous acid, butyl undecyl ester, Phthalic acid, octyl tridec-2-yn-1-yl ester etc. which have medicinal properties as mentioned in Table 1. The variety of biological roles indicates that multipurpose medicinal use of Apk Abraisham which ranges from antiseptic to hormonal regulation.

Conclusion

It could be summarized from the results and discussion that Apk abraisham does contain important biomolecules which provides a clue to its prescription for the ailments it is given. It will be of interest to probe into the medicinal roles of those compound present in Apk Abraisham for which the medicinal roles are not available.

Acknowledgements

The authors thankfully acknowledge the support of all the people and organizations involved in this project.

References

Author Info

Hassan Mohammad M1, Janaki CS2, Rao MRK3*, Prabhu K4, Deepa k5, Franklin6 and Vijayalakshmi N7

1Department of Anatomy, Northern Borders University, Arar, Saudi Arabia
2Department of Anatomy, Bhaarath Medical College, Chennai, Tamilnadu, India
3Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India
4Department of Anatomy, Sree Balaji Medical College and Hospital, Chennai, Tamil Nadu, India
5Department of Anatomy, Quest International University, IPOH Perak, Malaysia
6Department of Microbiology, CEO Anna Medical College, Mauritius, Montagne Blanche, Island, Mauritania
7Department of Chemical and Biotechnology, SASTRA Deemed-to-be-University, Thanjavur, Tamil Nadu, India
 

Citation: Hassan Mohammad M, Janaki CS, Rao MRK, Prabhu K, Deepa K, Franklin, Vijayalakshmi N,The Gas Chromatography Mass Spectroscopic Study of One Unani Drug, “Apk Abraisham”, J Res Med Dent Sci, 2022, 10 (8): 000-000.

Received: 09-Jun-2022, Manuscript No. JRMDS-22-57349; , Pre QC No. JRMDS-22-57349; Editor assigned: 11-Jun-2022, Pre QC No. JRMDS-22-57349; Reviewed: 25-Jun-2022, QC No. JRMDS-22-57349; Revised: 12-Aug-2022, Manuscript No. JRMDS-22-57349; Published: 24-Aug-2022

http://sacs17.amberton.edu/