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The Gas Chromatography Analysis of One Unani Drug, "Khustha Hajrul Yahood"

Journal of Research in Medical and Dental Science
eISSN No. 2347-2367 pISSN No. 2347-2545

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Research Article - (2022) Volume 10, Issue 8

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The Gas Chromatography Analysis of One Unani Drug, "Khustha Hajrul Yahood"

Hassan mohammad M1, Janaki CS2, Rao MRK3*, Prabhu K4, Deepa K5, Franklin6 and Vijayalakshmi N7

*Correspondence: Rao MRK, Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India, Email:

Author info »

Abstract

This work deals with gas chromatography mass spectrometric analysis of one Unani medicine, Khustha Hajrul Yahood, which is prescribed for urinary tract related ailments like urolithiasis, urinary tract infections, gonorrhoea etc. The medicine was bought from a Unani medicine shop and was processed suitably for gas chromatophraphy mass spectroscopic analysis. The results indicated the presence of many molecules such as Decane, 1-iodo-, Acetic acid, chloro-, decyl ester, 2-Isopropyl-5-methyl-1-heptanol, Sulfurous acid, butyl undecyl ester, Cyclohexane, 1-ethyl-1,3-dimethyl-, trans-, alpha-Santalol, Dodecanedioic acid, Methyl 2-hydroxy-octadeca-9,12,15-trienoate, Phthalic acid, hexadecyl hex-3-yl ester etc. which have far reaching medicinal roles, which could contribute to the medicinal role of Khushta Hazrul Yahood in treating urinary tract related ailments.

Keywords

GC MS, Khustha Hazrul Yahood, Urolithiasis, Gonorrhea, Alpha-Santalol, Sulfurous acid, Butyl undecyl ester

Introduction

Khushta Hajrul Yahood is a Unani medicine that is primarily used for the treatment of kidney diseases. It is also prescribed for urinary tract infections, gonorrhoea, gall stones etc. The ingredients are:

  • Hajrul–Yahood (Lapis judaicus)
  • Shora Qalmi (Potassium nitrate)
  • Aabe Mooli (Raphanus sativus)

Hajrul yahud (Lapis judaicus) is an also known as Jew's stone, which is a fossilised sea urchin spines. This medicine is used as a topical drug for pain, to improve urinary output and for prevent and treat kidney stones. Makbul et al. have reviewed the role of Hajrul yahud as a medicine for the management of urolithiasis [1].

Potassium nitrateis a used as medicine for small wounds and warts. Raphanus sativus is common edible plant which has many medicinal roles [2]. Torres et al. have elaborated the role of Raphanus sativus with reference to gall stones and control of cholesterol [3]. There are scanty reports on the role of Khushta Hajrul Yahood. It is imperative to establish the authenticity of alternative medicines such as Ayurveda, Sidhha and Unani systems as they are time tested and in use for centuries. The present workers have worked to scientifically evaluate the veracity of these medicine systems by latest techniques so that deeper knowledge of the mechanism of action of these medicines could be gained [4-22]. The present study in one step further in this endeavour.

Materials and Methods

Khushta Hajrul Yahood was bought from a Unani medicine vendor in Chennai. The medicine was suitably processed by standard procedures and the GC-MS analysis was performed.

Results

Khushta Hajrul Yahood GC MS profile and possible medicinal role of each molecule is tabulated in Table 1.

Ret. Time Molecule Mol. Formula Mol. Mass % Peak Area Possible Medicinal Role
3.69 Decane, 1-iodo- C10H21I 268.1 1.25 Idothyronine-deiodinase inhibitor
4.48 Acetic acid, chloro-, decyl ester C12H23ClO2 234.1 7.17 increases aromatic amino acid decarboxylase activity, Inhibits uric acid production
4.55 Cyclopentane, 1-butyl-2-propyl- C12H24 168.2 5.13 Not known
5.25 Dodecane, 1-fluoro- C12H25F 188.2 18.30 Not known
5.55 2-Isopropyl-5-methyl-1-heptanol C11H24O 172.2 1.43 Catechol-O-Methyl Transferase-Inhibitor,
5.69 Sulfurous acid, butyl undecyl ester C15H32O3S 292.2 1.22 Arachidonic acid inhibitor, increases aromatic amino acid decarboxylase activity, inhibits uric acid acid production
7.92 Cyclohexane, 1-ethyl-1,3-dimethyl-, trans- C10H20 140.2 1.73 Glutathione-S-Tansferase inhibitor, increases glutathinone –S-transferase (GST) activity, decreases oxaloacetate transaminase activity, Catechol-O-Methyl-Transferase-Inhibitor, Decrease Glutamate Oxaloacetate Transaminase, Decrease Glutamate PuruvateTransaminase, Glucosyl-Transferase inhibitor, increases glyoxalate transamination, reverse transcriptase inhibitor
8.88 .alpha.-Santalol C15H24O 220.2 1.19 5 alpha reductase inhibitor, HIF 1 alpha inhibitor, Ikappa B alpha phosphorylation inhibitor, increases alpha mannosidae activity, Interleukine 1 alpha inhibitor, TNF alpha inhibitor
13.79 cis-9-Hexadecenal C16H30O 238.2 1.21 Not known
14.30 Dodecanedioic acid C12H22O4 230.2 2.67 Arachidonic acid inhibitor, increases aromatic amino acid decarboxylase activity, inhibits uric acid production
16.82 9-Octadecenoic acid, (E)- C18H34O2 282.3 15.86 Not known
17.07 Methyl 2-hydroxy-octadeca-9,12,15-trienoate C19H32O3 308.2 10.88 17 beta hydroxysteroid dehydrogenase inhibitor, Aryl hydrocarbon hydroxylase inhibitor, testosterone hydroxylase inducer, Catechol-O-Methyl Transferase-Inhibitor
18.22 Phthalic acid, hexadecyl hex-3-yl ester C30H50O4 474.4 1.33 Asrachidonic acid inhibitor, increase aromatic amino acid decarboxylase activity, inhibits uric acid production Hexokinase stimulator

Table 1: Indicates the retentions values, types of possible compound, their molecular formulae, molecular mass, peak area and their medicinal roles of each compound as shown in the GC MS profile of Khushta Hajrul Yahood.

Figure 1 shows the graph profile of the Unani medicine Khushta Hajrul Yahood. The molecules were identified along with the possible pharmaceutical roles of each bio molecule was referred from National Agriculture Library, USA and others as shown in Table 1 [23].

medical-denta-science

Figure 1: indicates the GC MS profile of Khushta Hajrul Yahood.

Discussion

Khushta Hajrul Yahood showed some compounds like, Decane, 1-iodo-, Acetic acid, chloro-, decyl ester, 2-Isopropyl-5-methyl-1-heptanol, Sulfurous acid, butyl undecyl ester, Cyclohexane, 1-ethyl-1,3-dimethyl-, trans-, alpha-Santalol, Dodecanedioic acid, Methyl 2-hydroxy-octadeca-9,12,15-trienoate, Phthalic acid, hexadecyl hex-3-yl ester which have important medicinal roles as shown in Table 1. These medicinal roles could contribute to the urolithiatic effect of Khushta Hajrul Yahood.

Conclusion

It could be summarized from the results and discussion that Khushta Hajrul Yahood does contain important biomolecules which provides a clue to its prescription for the urolithiatic role of this medicine. It will be of interest to probe into the medicinal roles of many compound present in Khushta Hajrul Yahood for which the medicinal roles are not reported yet.

Acknowledgements

The authors thankfully acknowledge the support of all the people and organizations.

References

Author Info

Hassan mohammad M1, Janaki CS2, Rao MRK3*, Prabhu K4, Deepa K5, Franklin6 and Vijayalakshmi N7

1Department of Anatomy, Northern Borders University, Arar, Saudi Arabia
2Department of Anatomy, Bhaarath Medical College, Chennai, Tamilnadu, India
3Department of Anatomy, Amritha University, Thiruporur, Tamil Nadu, India
4Department of Anatomy, Sree Balaji Medical College and Hospital, Chennai, Tamil Nadu, India
5Department of Anatomy, Quest International University, IPOH Perak, Malaysia
6Deptartment of Microbiology, CEO Anna Medical College, Mauritius, Montagne Blanche, Island, Mauritania
7Department of Chemical and Biotechnology, SASTRA (Deemed to be University), Thanjavur, Tamil Nadu, India
 

Citation: Hassan mohammad M, Janaki CS, Rao MRK, Prabhu k, deepa k, Franklin, Vijayalakshmi N,The Gas Chromatography Analysis of One Unani Drug, “Khustha Hajrul Yahood”, J Res Med Dent Sci, 2022, 10 (8): 000-000.

Received: 09-Jun-2022, Manuscript No. JRMDS-22-57361; , Pre QC No. JRMDS-22-57361; Editor assigned: 11-Jun-2022, Pre QC No. JRMDS-22-57361; Reviewed: 25-Jun-2022, QC No. JRMDS-22-57361; Revised: 12-Aug-2022, Manuscript No. JRMDS-22-57361; Published: 24-Aug-2022

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