The Role of Agomelatine against Testicular Tissue Damage Due To Lipopolysaccharide-Induced Sepsis in Rats
Author(s): Arzu Yalcin*, Oğuzhan Kavrık, Ulker Tunca, Mustafa Saygin and Ozlem Ozmen
Abstract
Background: We investigated role of Agomelatine (AGO) in the sepsis model. Materials and Methods: 24 male Wistar rats (12-month-old, 250-300 g) were used in the study. The study consists of 3 groups. Control group: 0.1 ml normal saline by gavage (single dose) ± 0.1 ml normal saline intraperitoneal (i.p, single dose) to right inguinal region of the rats. LPS group: 0.1 ml normal saline by gavage (single dose) + 5 mg / kg LPS (single dose) i.p to right inguinal region. LPS+AGO group: 5 mg / kg LPS (single dose) i.p to right inguinal region+20 mg/kg AGO (by gavage, single dose, 30 min before the LPS administration). Results: In the testis tissue in LPS+AGO, catalase expression was significantly increased as opposed to that of the control and LPS groups (p<0.05). When MDA activity was analyzed, LPS+AGO group showed a statistically significant decrease compared to LPS and control groups (p<0.05). Diffuse hyperemia, expressions of Caspase-8, Haptoglobin, IL-4, IL-10 were increased in LPS group according to control group (p<0.001). Expressions of Caspase-8, Haptoglobin, IL-4, IL-10 were decreased in AGO-treated group compared to LPS group (p<0.001). Sirtuin-1 expression was increased in AGO-treated group according to LPS group (p<0.001). Conclusion: AGO may reduce sepsis-induced inflammation.