Polyphenolic Compounds from Lactuca serriola Aerial Parts Growing in Iraq Exhibit Anticancer Properties and Concurrent HPLC-DAD Profiling
Author(s): Murtadha Saleh Hussien, Ayad A Al-Hamashi*
Abstract
The present study was carried out to investigate the phytochemical constituent of Iraqi Lactuca serriola aerial part and to evaluate the cytotoxic effect of plant. The aerial part of Lactuca serriola were collected, dried, pulverized, and extracted with methanol using Soxhlet. The extract was concentrated, and the resulting residue was successively partitioned with solvent have different polarity including hexane, chloroform, ethyl acetate, N-butanol. The result fractions were subjected to general phytochemical screening, thin layer chromatography and High Performance Liquid Chromatography (HPLC) analysis. The results indicated the presence of phytosterol in the hexane fractions and the phenolic substances in the other fractions. Quercetin, chlorogenic acid, caffeic acid, lactucin, rutin, and quercetrin are six active constituents that were identified from Iraqi Lactuca serriola's n-butanol fraction. In vitro testing for cytotoxicity against AMJ13 breast cancer cell line revealed that the aerial parts of the plant Lactuca serriola of the Asteraceae family contained an ethyl acetate extract that has a promising cytotoxicity against breast cancer. According to the research, Lactuca has 67.3% cytotoxic activity at the tested dose of 100 mg/mL against the AMJ13-inhibited cancer cell.