Green Synthesis, Characterization and Assessment of Anti-inflammatory and Antioxidant Properties of Silver Nanoparticles Prepared using Amla Extracts-An In-vitro Study
Author(s): Divij Khullar* and Nabeel Ahmed
Abstract
Toxic chemicals are used in traditional nanoparticle synthesis processes, resulting in environmental toxicity. As a result, we must switch to "green synthesis." Previous studies have shown that Silver nanoparticles reinforced with Amla extract have excellent antimicrobial properties. As a result, this research was carried out to determine the anti-inflammatory and antioxidant properties of Silver. Since Silver induces cell wall lysis due to intracellular material leakage, it is an excellent antimicrobial agent and was chosen to make a nanoparticle. Aim: Aim of the study was phyto assisted preparation of Silver nanoparticles from Amla fruit and evaluation of its anti-inflammatory and antioxidant properties. Material and methods: Anti-inflammatory and antioxidant properties of the nanoparticle were assessed using Bovine Serum Albumin (BSA) and DPPH Assay respectively at 10 µL, 20 µL, 30 µL, 40µL, 50 µL. Results: Values for anti-inflammatory property of nanoparticles were higher than the standard values at 40µL, 50 µL concentrations. Percentage of inhibition was highest at 40 µL (86%) and 50 µL (84.6%). The values for antioxidant property of nanoparticles were found to be higher than the standard values at concentrations except at 40 µL, 50 µL. Percentage of inhibition was highest at 20 µL (86.2%) Conclusion: Within the limits of the study it can be concluded that Silver nanoparticles have exceptional anti-inflammatory and antioxidant properties and further can be incorporated in dental material or can be used to coat suture materials to improve their properties.