Evaluation of interleukin-35 role in fixed orthodontics patients
Author(s): Asaad Kareem Al-Nafaee*, Rasha Jasim Alwarid and Kasem Ahmed Abeas
Abstract
During orthodontic treatment, the immune system targets the teeth through inflammatory processes. Immunological alterations in the gingival crevicular fluid and saliva might occur following fixed orthodontic appliance bonding. Evaluation of Interleukin -35 levels in saliva during initial fixed orthodontic treatment for limited age groups at different time intervals. A total of eighty-eight samples were obtained from saliva (unstimulated) 2–3 mL was collected. The range of ages, from 14 to 35 years from Babylon City, Iraq was included in the case study. during the same time span of study specimen collection from November (2021) to April (2022). The samples were classified into two groups, including 68 patients (orthodontic) as well as a control group of 20 (non-orthodontic). The enzyme-linked immunosorbent assay was used to determine the IL-35 level in saliva. The mean ± SD of IL-35 in orthodontic (patients ) and non-orthodontic (control group) is 13.47 ± 11.22 pg/mL. This value is high significant with less than 0.01 according to independent T-test. There was significant difference in interleukin-35 concentration in <1, 1-2, 2-4, and >5 month was (8 ± 1.41; 19.8 ± 7.9; 32.1 ± 10.3; 41 ± 12.2) pg/mL, comparing with it is concentration in control groups (5.9 ± 2.4) pg/mL. It is concluded that the level of IL-35 saliva in fixed orthodontic patients is higher than that of control group (non- orthodontic).