Effectiveness of Different Agents for Disinfection of Gutta Percha Cones an in vitro study
Author(s): Madhuri Agrawal*, Ganesh Kotalwar, Akshay Gelda, Safalya Kadtane, Abhishek Badade and Vivek Hegde
Abstract
Aim: The aim of the present study was to evaluate and compare the 1) sterilizing effect of different disinfecting solutions on gutta-percha cones: 5.25% sodium hypochlorite, 2% chlorhexidine gluconate, propolis and ozonated water.2) to find out the minimum time required to effectively disinfect gutta -percha cones.
Method: Fifty size 30 gutta-percha cones were used in this study. 40 cones were randomly divided into 4 groups of 10 samples each.5 cones served as positive control & 5 cones served as negative control. The cones were contaminated by immersion in saliva and then were disinfected by immersing in the above mentioned four disinfecting solutions: 5.25% sodium hypochlorite (G1), 2% chlorhexidine gluconate (G2), propolis (G3) and ozonated water (G4) for periods of 30 seconds and 60 seconds. After the disinfection procedure, the cones were incubated in brain heart infusion broth for 72hrs and the presence of bacterial growth was analysed by turbidity of the medium. Data was analysed by Fisher’s exact test.
Results: 2% chlorhexidine gluconate(G2) at both times evaluated did not show bacterial growth.5.25% sodium hypochlorite(G1) showed growth after immersion for 30 sec but after 60 sec did not show bacterial growth. Propolis (G3) and Ozonated water (G4) showed diverse results after the immersion for 30 sec and 60 sec.
Conclusion: Immersion of GP cones in 2% chlorhexidine gluconate for 30sec was an effective method for GP disinfection, while 5.25% sodium hypochlorite needed 60 sec of immersion to disinfect the GP. Ozonated water can be used for disinfection of gutta-percha cones. Propolis is less effective for disinfection of gutta percha cones.