Detecting the Alteration of Vaspin, Insulin and Some Biochemical Parameters in Blood of Type2 Diabetic Patients
Author(s): Zahraa Abdalelah Saleem and Khalid Shaalan Sahab*
Abstract
Background: T2DM is the most prevalent metabolic disease in the world and is characterized by defects in insulin secretion and a peripheral insulin resistance in the skeletal muscle, the adipose tissue and the liver. The current study aimed to estimation of Vaspin and some biochemical parameters included insulin, fasting blood glucose (FBG), HbA1c, urea, creatinine, lipid profile [Triglyceride (TG), Total Cholesterol (TC), High-density lipoprotein (HDL), low density lipoprotein (LDL), Very low density lipoprotein (VLDL)] and C-reactive protein (CRP)in blood of patients suffering from type2 diabetes mellitus, and detecting the change of the ratio in patients comparing with the healthy people. Methods: Sixty patients with type2 diabetes mellitus recruited from Baquba Teaching Hospital/ Diyala governorate in Iraq during the period 1/10/2021 to 30/12/2021. 30 healthy individuals were included as a control. The ELISA Kit was used to estimation serum vaspin and insulin. The blood FBG, HbA1c, TG, TC, HDL, Urea, Creatinine and CRP tests were assessed using an available commercially laboratory kits. Results: The concentration of serum vaspin inT2DM patients group was significantly lower than that of the healthy subjects (p<0.05). The concentration of serum insulin in patients was higher than of healthy subjects but without significant differences (p>0.05). There were a significant difference in the serum FBG, HbA1c and urea levels in patients compared to controls (p<0.05), while creatinine concentration increased no significantly in patients compared to controls (p>0.05). CRP showed significant differences between studied groups. The levels of TC, TG, LDL and VLDL were higher significantly in patients compared to controls (p<0.05), while HDL was decreased non-significantly in patients compared to controls. There were no significant connections between serum Vaspin and insulin concentrations with FBG, HbA1C, CRP, urea, creatinine, TC, TG, LDL, HDL, and VLDL levels or with anthropometries measurements. Vaspin had lowest area under curve (AUC) and sensitivity in ROC curve Conclusion: Vaspin concentrations were decrease in plasma of patients of T2DM. Vaspin levels were significantly lowest in patients of T2DM and had the lowest AUC and sensitivity in ROC curve. Vaspin is not correlated with any studied parameter. Therefore, the decreasing significantly in serum vaspin level may be a probable indicator of the worsening of T2DM.