Antibacterial and antimicrobial activity of Lactobacillus plantarumisolated from cow milk against bacterial pathogen isolated from burns infection
Author(s): Rawan Raad Hussien* and Ammar Algburi
Abstract
Burns infection is one of the most common consequences and the leading causes of death. The burn infections are mostly contaminated with bacterial species resistant to several types of antibiotics. This study comes to evaluate a safe and alternative antimicrobial in order to control the multi-drugs resistant (MDR) isolates. In this study, the minimum inhibitory concentrations (MIC) and minimum biofilm inhibitory concentrations (MBIC) of cell free supernatants prepared from Lactobacillus plantarumisolates (CFS-LP) were determined against the most bacterial contaminants isolated from burn infections. Forty two of cow's milk samples were collected from different areas in Baghdad, Iraq. In addition, 187 swabs of burns infection were collected from patients admitted to the Burns Specialized Hospital/Medical city in Baghdad. We reported that Pseudomonas aeruginosa (34.22%) and Staphylococcus aureus (27.27%) were the most predominant MDR isolates. L. plantarum spp. 2 was more effective than the other Lactobacilli isolates. The MIC90 of CFS-LP2 ranged from 25%-50%, preventing more than 90% of the bacterial contaminants growth. In addition, the MBIC50 of CFS-LP1, 2 and 3was in rang (6.25%-25%), inhibiting more than 50% of biofilm formed by burns infection isolates. The authors concluded that CFS of L. plantarumisolated from cow’s milk is effective and safe substances could be used to control the pathogenic bacterial caused burns infection and their biofilm formation.