Antibacterial Activity and Cytotoxic Effect of Bisphosphonate Conjugated Gold Nanoparticle Synthesized using Asparagus racemosus Root Extract
Author(s): Sruthi H*, Navaneethan R and Rajeshkumar S
Abstract
The nanoparticles can be surface modified to attach a variety of ligands which enable them to be biosensors, molecular-scale fluorescent tags, imaging agents, and targeted drug delivery vehicles. Green synthesis of nanoparticles is a type of bottom-up approach in which reaction occurs with the help of reducing and stabilizing agents. Bisphosphonates are increasingly being used to treat a wide range of skeletal issues, including hereditary skeletal disorders in children, cancer-related bone metastases and osteoporosis. Asparagus racemosus is a green edible medical plant used in Ayurvedic preparations to treat aging, vigor, immunity, longevity, and skeletal issues. It has been shown to have a variety of pharmacological properties, including antioxidant, antimicrobial, anti-inflammatory, and antiseptic properties. Bisphosphonate conjugated with gold nanoparticle have shown to produce more targeted action. The goal of this study was to green synthesise Bisphosphonate conjugated gold nanoparticles with asparagus racemosus extract and to check their antimicrobial activity and cytotoxicity. Green synthesis of Bisphosphonate conjugated gold nanoparticles using Asparagus racemosus extract was achieved and is characterized. The present study revealed that green preparation of gold nanoparticles is achievable in a simple and eco-friendly manner using Asparagus racemosus root extract. The gold nanoparticles were assessed for their Antibacterial activity against S. mutans, Lactobacillus, and C. albicans. Cytotoxicity of the nanoparticles was assessed using Brine Shrimp Lethality Assay. Gold nanoparticles have good antibacterial activity against Streptococcus mutants (150 ugs/ml–17 mm zone of Inhibition) & Lactobacillus (150 ugs/ml–20 mm zone of Inhibition) and showed 20% lethality at 50ul concentration.