Antianxiety Activity of Ethanolic Extract of Triticum aestivum in Acute Stress Induced Wistar Albino Rats by Light and Dark Apparatus
Author(s): Swapna Mahapatra, Prasanta Kumar Nayak, Subrat Kumar Sahany and Saroj Shekhar Rath*
Abstract
Objectives: The present study was designed to evaluate the antianxiety activity of Ethanolic extract of Triticum aestivum (TAE) on acute stressed wistar albino rats. Materials and methods: Effect of TAE was studied on acute restraint stress induced rats. The reference standard drug (Diazepam 1mg/kg po) and the test drug, TAE at doses of 150mg/kg and 200mg/kg b.w. were given to rats for 14 days. Anti-anxiety activity was assessed by using Light and Dark Box test. Animals sacrificed at the end of this experiment. Then the body weight, adrenal and spleen weight, ulcer index as well as various biochemical parameters like Malondialdehyde (MDA) and Superoxide dismutase (SOD) were assessed. Results: Both Diazepam and TAE-200 mg/kg treated rats showed a significant prolonged stay in the light box when exposed to acute stress and this effect was comparable to that of normal no stressed rats. Conclusion: The test drug, TAE-200mg/kg shows anxiolytic activity against acute stress in wistar albino rats.